Séminaire du laboratoire PhLAM par Kevin BRAECKMANS

Ce séminaire sera animé par Kevin BRAECKMANS, Professeur à l'Université de Gand, et aura pour thème "Photoporation: leveraging light and nanoparticles for gentle, high-throughput cell transfections".

Résumé :

Delivery of bioactive compounds, such as proteins and nucleic acids, into cells in vitro or ex vivo is a generic requirement for many applications in the life sciences, such as for the engineering of therapeutic cells. Physical delivery methods are attractive in this context as they are well-controlled, and can accommodate a broad variety of effector molecules and cell types. Photoporation is such a recently developed physical delivery technology which combines laser stimulation with photothermal nanoparticles. Localized thermal effects upon laser irradiation can create pores in the cell membrane, allowing the influx of external molecules in cells. Importantly, photoporation is very gentle to cells, resulting in excellent cell viability and preservation of a cell’s phenotype and functionality. In this presentation I will give an introduction to the concept of photoporation, followed by an overview of the most notable work that we performed on the development and application of this technique to biomedical problems. Finally, I will highlight how this research has led to the establishment of the spin-off company Trince and give an update on its current status.

Le résumé est disponible ici.